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Nanocascade Engineering Workshop for Synergistic Microenvironment Reprogramming and EpSC Revitalization in Precise Diabetic Wound Therapy - PubMed

3 hours ago
  • #nanocascade engineering
  • #microenvironment reprogramming
  • #diabetic wounds
  • Chronic diabetic wounds are characterized by impaired closure due to chronic inflammation and impaired regeneration.
  • Poor proliferative and differentiative ability of epidermal stem cells (EpSCs) in an inflammatory microenvironment delays healing.
  • A nanocascade engineering workshop (Cu5.4O@LL-37/pDNA) was designed to reshape the inflammatory microenvironment and activate EpSC functions.
  • The core of the workshop is an ultrasmall Cu5.4O nanozyme that eliminates reactive oxygen species and enhances the inflammatory response.
  • The shell is constructed from plasmid DNA (pDNA) and antibacterial peptide LL-37, improving gene transfection efficiency and inhibiting bacterial infection.
  • The system improves gene delivery and sustains P311 expression, promoting EpSC proliferation and differentiation.
  • This nanotherapy accelerates re-epithelialization and wound closure in diabetes and infection models.
  • The treatment strategy offers a novel approach for durable and effective healing of chronic wounds.