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Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemia - PubMed

4 hours ago
  • #bispecific antibodies
  • #T-cell engagement
  • #acute myeloid leukemia
  • The study addresses challenges in T-cell engaging bispecific antibodies (TCEs) for acute myeloid leukemia (AML), including lack of tumor-selective targets and insufficient T-cell activation.
  • A CD33xCD28 IgG4-scFv2 antibody was created to provide CD28 co-stimulation and tested in combination with a CD117xCD3 TCE.
  • The combination enhanced T-cell mediated lysis of AML cells by improving T-cell activation, proliferation, cytokine release, attachment speed, and lytic events.
  • This approach also increased specificity towards AML cells expressing both CD117 and CD33 targets, potentially reducing off-target effects.