Inducible, split base editors for in vivo cancer functional genomics - PubMed
2 hours ago
- #cancer functional genomics
- #CRISPR base editors
- #in vivo screening
- Researchers developed small-molecule-controllable split-engineered base editors (seBEs) to reduce cellular toxicity and enable inducible in vivo functional genomics screens in cancer.
- High-density seBE screens using thousands of guide RNAs identified known and novel loss-of-function and dominant-negative mutations in cancer therapeutic targets.
- A deep tiling seBE screen against Adar1 revealed critical residues that show distinct non-cell-autonomous cancer dependencies in vivo but not in vitro, highlighting the system's utility for uncovering key functional residues.