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Dietary carboxymethylcellulose metabolite promotes heart allograft rejection through induction of pro-inflammatory macrophages via lysophosphatidic acid - PubMed

3 days ago
  • #Carboxymethylcellulose
  • #Heart transplant rejection
  • #Macrophage polarization
  • Dietary carboxymethylcellulose (CMC) promotes heart allograft rejection by inducing pro-inflammatory macrophages via lysophosphatidic acid (LPA).
  • CMC-fed mice showed increased LPA-producing bacteria and higher serum LPA levels, leading to transplant rejection.
  • Fecal microbiota transplantation and gut microbiota depletion confirmed the role of gut microbiota in CMC-induced rejection.
  • LPA-treated macrophages accelerated allograft rejection by upregulating glycolysis, while glycolysis inhibitors promoted allograft survival.
  • Human transplant rejection patients had elevated serum LPA levels and LPAR6 expression in graft-infiltrating macrophages.
  • LPA promoted LPAR6 expression, activating the mTOR/HIF-1α pathway to enhance glycolysis and pro-inflammatory macrophage polarization.
  • Treatment with LPAR antagonist Ki16425 prolonged allograft survival in CMC-fed recipients.