Crosslinked ionizable lipids reprogram dendritic cell metabolism for potent mRNA vaccination - PubMed
4 hours ago
- #metabolic reprogramming
- #lipid nanoparticles
- #mRNA vaccines
- Developed a lipid nanoparticle (LNP) for metabolic reprogramming of dendritic cells in mRNA vaccines.
- Designed a crosslinked ionizable lipid, C12-2aN, using imidoester-based conjugation chemistry.
- C12-2aN promotes mRNA expression and stimulates glycolysis via mTORC2 pathway activation.
- Demonstrated potent vaccine efficacy in SARS-CoV-2 and OVA cancer vaccine models.
- C12-2aN LNPs showed reduced off-target delivery and lower immunogenicity compared to FDA-approved LNPs.
- Integration of mRNA delivery and metabolic reprogramming offers potential for next-gen mRNA LNP vaccines.