Candida auris vacuolar calcium pump mediates fluconazole efflux and resistance evolution - PubMed
5 days ago
- #Candida auris
- #CDT1
- #fluconazole resistance
- Candida auris is an emerging fungal pathogen with high intrinsic resistance to fluconazole.
- A pooled screen of piggyBac transposition mutants identified mitochondrial genes linked to reduced fluconazole susceptibility.
- The vacuolar calcium pump homologue CDT1 (Calcium and Drug Transporter 1) was found responsible for reduced fluconazole susceptibility.
- Fluconazole induces upregulation of CDT1 via the calcineurin signalling pathway.
- Cdt1 mediates fluconazole efflux through its plasma membrane localization, dependent on calcineurin and ATP hydrolysis.
- Cdt1 accelerates the evolution of fluconazole resistance or tolerance and is elevated in resistant clinical isolates.
- The study reveals a neofunctionalized role for Cdt1 in fluconazole efflux in Candida auris.