LC3-linked Golgi-bypass exocytosis fortifies epithelial barrier defense - PubMed
5 hours ago
- #exocytosis
- #LC3
- #epithelial barrier
- The study identifies a conserved exocytic pathway called LC3/Atg8-associated carrier exocytosis (LACES), which links microbial signals to rapid surface protein delivery.
- In the C. elegans intestine, PCN triggers VPS-34-dependent PtdIns3P production, leading to Atg8ylation on RAB-8 carriers, speeding up ABC transporter PGP-1 delivery and improving infection survival.
- LACES operates independently of the unfolded protein response, autophagy initiation, and LC3-associated phagocytosis, and is activated by various bacteria.
- In mammalian epithelia, PCN enhances apical delivery of ΔF508-CFTR in Caco-2 cysts with functional gains and strengthens LC3-RAB8 interactions in mouse intestines, reinforcing barrier defense.