Single-cell transcriptomics identifies NF-κB/STAT3-associated neutrophil inflammatory reprogramming in rheumatoid arthritis synovium - PubMed
3 hours ago
- #NF-κB
- #Neutrophils
- #Rheumatoid arthritis
- Single-cell RNA sequencing was performed on 8 RA patients (66,539 cells) to study neutrophil heterogeneity in rheumatoid arthritis synovium.
- Infiltrating neutrophils showed inflammatory C1 (Chemokine+) and C3 (S100A8/A9+) phenotypes.
- Co-expression analysis identified functional modules (M3/M4) enriched in NF-κB and type I interferon signaling.
- Neutrophils influenced the synovial microenvironment via CXCL, IL1, and MIF communication pathways.
- The NF-κB/STAT3 axis was identified as a regulatory node, with NFKB1 affecting S100A8/A9 effector genes.
- Findings were validated in an independent bulk RNA-seq cohort (n = 19), showing correlation with systemic inflammatory markers (ESR/CRP).