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FineST: contrastive learning integrates histology and spatial transcriptomics for nuclei-resolved ligand-receptor analysis - PubMed

3 hours ago
  • #cell-cell communication
  • #spatial transcriptomics
  • #contrastive learning
  • FineST is a deep contrastive learning model integrating histology and spatial transcriptomics (ST) for nuclei-resolved ligand-receptor analysis.
  • It addresses limitations of ST, such as low resolution and data sparsity, by leveraging histology images for precise nuclei segmentation and high-resolution RNA imputation.
  • FineST outperforms existing methods in RNA imputation, cell type prediction, and cell-cell communication (CCC) pattern discovery.
  • Applied to colorectal and breast cancer datasets, it reveals novel tumor-immune interactions, including invasive fronts, tertiary lymphoid structures, and therapy resistance barriers.
  • The study highlights a new paradigm in ST analysis by integrating histology images, enhancing CCC insights across cancer types.