Proteome Profiling Reveals Inflammation and Fibrosis Biomarkers in Urinary Migrasomes of Patients with Diabetic Kidney Disease - PubMed

8 hours ago

The study used Astral-DIA proteome to profile urinary migrasomes and serum from diabetic kidney disease (DKD) patients, identifying potential biomarkers for disease status.
Key proteins like LAMA1, ITGB3, FGG, and FGB showed interactions in differentially expressed proteins, with ITGB3 and FGB linked to inflammation and fibrosis pathways.
Urinary migrasomes likely originate mainly from podocytes and partially from monocytes/macrophages, and contained proteins such as NLRC4, ITGB3, and FGB.
In experiments, umig from DKD patients stimulated HK2 cells to increase expression of inflammatory markers like TLR4, p-NF-κB p65, NLRC4, caspase-1 p20, IL-1β, and TNF-α, indicating umig promote inflammation via the TLR4-NF-κB pathway.
Elevated levels of NLRC4, ITGB3, and FGB were found in renal tissues of DKD patients and db/db mice, correlating with inflammation and fibrosis.
The abundance of NLRC4, ITGB3, and FGB in urinary migrasomes suggests their potential as noninvasive biomarkers for monitoring inflammation and fibrosis in DKD.

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