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Synergistic immunotherapeutic effects of irreversible electroporation and CAR-NK cell therapy against hepatocellular carcinoma - PubMed

3 days ago
  • #immunotherapy
  • #hepatocellular carcinoma
  • #CAR-NK cells
  • CAR-NK cell therapy is a promising immunotherapeutic approach with strong cytotoxicity and safety.
  • Hepatocellular carcinoma (HCC) has a suppressive tumor microenvironment (TME) that limits CAR-NK cell efficacy.
  • Irreversible electroporation (IRE) reshapes the TME by inducing immunogenic cell death and releasing chemokines like CX3CL1.
  • IRE-treated HCC cells show increased susceptibility to NK-mediated cytotoxicity due to elevated reactive oxygen species.
  • Combining IRE with GPC3-targeted CAR-NK cells enhances tumor lysis in vitro and tumor regression in HCC models without systemic toxicity.
  • The study proposes a clinically actionable treatment paradigm for overcoming resistance in solid tumors like HCC.