Optimizing linker length of base editors for precise crop breeding and gene therapy - PubMed
4 hours ago
- #Crop breeding
- #Gene therapy
- #Base editing
- Base editing allows efficient nucleotide conversions without DNA double-strand breaks or donor templates.
- Bystander editing is a risk due to broader editing windows, leading to unintended mutations.
- Linker length significantly affects the editing window; a 7-amino-acid linker reduces bystander editing by 54.4%.
- Optimized linker length strategy improves specificity for crop breeding and gene therapy.
- Shortening the linker enhances base editing specificity, addressing safety concerns in agriculture and therapy.