Profiling presynaptic scaffolds using split-GFP reconstitution reveals cell-type-specific spatial configurations in the fly brain - PubMed
3 hours ago
- #D. melanogaster
- #synapse profiling
- #neuroscience
- Characterization of synapse heterogeneity aids in understanding neuronal circuits.
- Split-GFP reconstitution visualized endogenous Bruchpilot (Brp) in a cell-type-specific manner.
- High-throughput quantification pipeline profiled AZ structures in mushroom body circuit neurons.
- Quantitative analysis revealed cell-type-dependent synaptic heterogeneity and stereotypy.
- Discovered sub-compartmental synapse configuration and transient structural plasticity from associative learning.
- Profiles show multilayered spatial configurations of AZs, from overall distribution to local arrangements.