Phase separation of heterochromatin protein 1 regulates virulence gene expression in Plasmodium falciparum - PubMed
4 hours ago
- #heterochromatin
- #phase separation
- #Plasmodium falciparum
- Phase separation of PfHP1 regulates virulence gene expression in Plasmodium falciparum.
- PfHP1 undergoes phase separation in vitro and compartmentalizes DNA, tunable by RNA and PfH2A.Z.
- Single-molecule assays show PfHP1 preferentially compacts AT-rich DNA.
- Point mutations in PfHP1's disordered region disrupt phase separation and DNA compaction.
- Phase-separation-defective mutants cause dispersed nuclear localization and derepression of var genes.
- Hi-C analysis shows loss of heterochromatic interactions, linking PfHP1 phase separation to var gene silencing.