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Drosophila melanogaster miPEP8 regulates cell size through its interaction with ref(2)P/p62 - PubMed

4 hours ago
  • #cell size regulation
  • #autophagy
  • #Drosophila melanogaster
  • Drosophila melanogaster miPEP8 regulates cell size via interaction with ref(2)P/p62.
  • miPEP8 overexpression in S2 cells reduces cell size, increases G1 phase proportion, and decreases autophagic flux.
  • Proteomics shows miPEP8 upregulates ref(2)P/p62, linking it to mTORC1/autophagy pathway.
  • A short linear motif (SLiM) in miPEP8 is crucial for its interaction with ref(2)P/p62.
  • Mutation of SLiM or RNA interference of ref(2)P/p62 reverses the cell size phenotype.
  • In vivo studies confirm miPEP8's role in wing size regulation in Drosophila.