Microtubules guide Aurora B substrate geometries for accurate chromosome segregation - PubMed
3 hours ago
- #Aurora B
- #Chromosome Segregation
- #Microtubules
- Microtubules regulate Aurora B phosphorylation of substrates for accurate chromosome segregation.
- A new cryo-electron microscopy workflow reveals microtubule-bound structures of the CPC and Aurora B substrates.
- Microtubules can either promote or restrict Aurora B-mediated phosphorylation based on substrate binding geometry.
- The Ndc80 complex oligomerizes on microtubules, restricting kinase access to prevent phosphorylation-induced detachment.
- Attenuating Ndc80 oligomerization leads to unstable kinetochore-microtubule attachments and chromosome mis-segregation.
- Microtubule-bound CPC promotes phosphorylation and inactivation of MCAK, a microtubule-depolymerase.
- Microtubule-guided substrate remodeling is a general mechanism for controlling Aurora B phosphorylation during mitosis.