Phase separation of Rht8-derived RNHL1 integrates ethylene and gibberellin signaling to regulate wheat internode elongation - PubMed
5 hours ago
- #Phase separation
- #Wheat
- #Plant height regulation
- Rht8, a semi-dwarfing allele in wheat, encodes RNHL1 (Ribonuclease H-Like 1), regulating plant height through liquid-liquid phase separation (LLPS).
- RNHL1 forms nuclear biomolecular condensates via intrinsically disordered regions (IDR1 and IDR3) and interacts with ethylene signaling transcription factor TaEIL1.
- RNHL1-TaEIL1 condensates repress TaERF1, which in turn suppresses gibberellin (GA) biosynthetic gene TaGA3ox2, modulating GA-mediated internode elongation.
- Genetic analyses show RNHL1 and TaEIL1 positively regulate plant height, with loss-of-function mutants exhibiting dwarf phenotypes.
- The study reveals an integration point between ethylene and GA signaling orchestrated by RNHL1-TaEIL1 phase separation, offering insights for wheat improvement.