Ki-67 shapes the nucleolus by anchoring chromatin via its amphiphilic properties - PubMed
6 hours ago
- #nucleolus
- #Ki-67
- #chromatin anchoring
- Ki-67 regulates nucleolar shape by anchoring chromatin at the chromatin-nucleolus interface through its amphiphilic properties.
- Depletion of Ki-67 leads to nucleolar rounding and reduced chromatin enrichment at the nucleolar rim and within internal invaginations.
- The amphiphilic properties of Ki-67 are conferred by two distinct affinity domains separated by a spacer, driving chromatin anchoring.
- Rounded nucleoli are associated with chromatin loss, and acute chromatin digestion also induces nucleolar rounding, indicating chromatin environment is key for shape.
- The study identifies a novel Ki-67-mediated chromatin anchoring mechanism linking nucleolar morphology to genome organization and condensate morphology.