SNX-mediated biogenesis of a plant-unique vesicle derived from the multivesicular body - PubMed
6 hours ago
- #SNX proteins
- #retrograde transport
- #plant cell biology
- Plant retrograde transport is mediated by a novel class of spherical vesicles (30-50 nm) derived from multivesicular bodies (MVBs).
- These MVB-derived vesicles are enriched with retromer components and vacuolar sorting receptors (VSRs), indicating their role as retrograde carriers.
- Arabidopsis SNX1 forms shorter membrane tubules than mammalian SNX1, and SNX1-SNX2 heterodimers can generate spherical vesicles similar to those observed in vivo.
- Knockdown of SNX1 or SNX2 leads to vacuolar mislocalization and increased degradation of GFP-VSR2, impairing retrograde transport.
- Defects in SNX1 and VPS29 inhibit the formation of these spherical vesicles, causing embryonic lethality before the globular stage in Arabidopsis.