VGLL4-driven TEAD4 multimerization orchestrates DNA binding and YAP recruitment - PubMed
3 hours ago
- #TEAD4 multimerization
- #Hippo pathway
- #transcription regulation
- VGLL4 promotes TEAD4 multimerization, enhancing its DNA binding stability and YAP recruitment.
- Monomeric TEAD4 binds to DNA with fast association and dissociation rates, but multimerization increases DNA residence time.
- Both YAP and VGLL4 can induce TEAD4 multimerization, strengthening DNA binding and sequence specificity.
- VGLL4 has a stoichiometry-dependent effect on YAP recruitment: low ratios enhance it, while high ratios inhibit it.
- The study uses fluorescence-combined optical tweezers to reveal dynamic TF-DNA interactions in the Hippo pathway.