Single-Cell Transcriptomics Reveals FLS2-Dependent Hypoxia Signaling and ERF13-Mediated Transcription During flg22-Triggered Immunity - PubMed
4 days ago
- #FLS2
- #Hypoxia signaling
- #ERF13
- FLS2-dependent immune responses to flg22 in Arabidopsis were studied using single-cell RNA sequencing.
- Epidermal and mesophyll cells showed FLS2-dependent transcriptional changes during immune activation.
- flg22 induced a hypoxia-like response, with mutants in hypoxic signaling showing reduced sensitivity to flg22.
- ERF13 was identified as a key regulator, balancing immunity and growth responses.
- ERF13 mutants impaired flg22-triggered ROS and growth inhibition but enhanced effector-triggered immunity (ETI).
- flg22 altered ploidy and cell-cycle gene expression, with stabilization observed in ate1 and erf13 mutants.
- Cell-cycle mutants showed varied sensitivity to flg22, indicating crosstalk between immune and cell-cycle pathways.
- Immune, hypoxia, and ROS signals converge via ERF13 to regulate stress adaptation and immunity.