iCLAP: an innovative method for integrable co-detection of low-abundance antigens with high-plex immunostaining - PubMed
4 hours ago
- #FFPE tissues
- #multiplex imaging
- #spatial proteomics
- iCLAP is an innovative method for integrable co-detection of low-abundance antigens with high-plex immunostaining.
- It enables sensitive and highly multiplexed protein detection in formalin-fixed, paraffin-embedded (FFPE) tissues.
- The method combines iterative signal amplification with efficient fluorophore inactivation for repeated staining.
- iCLAP can profile more than 40 markers, expanding analytical capabilities of FFPE tissues.
- Application in human pancreatic tissues revealed spatially distinct senescence-associated protein patterns.
- The technique supports high-sensitivity, high-dimensional spatial proteomic studies of complex biological processes.