Unraveling pancreatic ductal adenocarcinoma at single-cell resolution with spatial insights: From mechanisms to clinical translation - PubMed
4 hours ago
- #Single-cell RNA sequencing
- #Pancreatic cancer
- #Tumor microenvironment
- Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with significant cellular heterogeneity and a dense desmoplastic stroma.
- Single-cell RNA sequencing (scRNA-seq) has advanced understanding of PDAC by detailing its cellular composition and revealing complex tumor microenvironment (TME) interactions.
- The TME in PDAC involves immune evasion, metastatic progression, and therapeutic resistance, driven by interactions among T cells, myeloid populations, fibroblasts, and malignant cells.
- This review summarizes scRNA-seq studies on PDAC heterogeneity, tumorigenesis, immune remodeling, therapeutic resistance, and biomarker discovery.
- Integrative single-cell and spatial multi-omics approaches are discussed to map the TME, offering insights into PDAC biology at single-cell and spatial resolution.