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Standardized chronic restraint stress protocols reveal dynamic evolution of behavioral adaptations in male mice: implications for translational neuroscience - PubMed

10 hours ago
  • #behavioral neuroscience
  • #chronic stress
  • #translational research
  • Short-duration, high-intensity CRS (6h/day for 3 days) induces persistent avoidance-related and repetitive behaviors in male mice.
  • Prolonged CRS (2h/day for 10-14 days) leads to progressive deficits in reward-seeking and behavioral coping, transitioning from initial avoidance/repetitive behaviors.
  • The 10-day CRS protocol is critical for inducing comorbid avoidance and reward-processing impairments, serving as a model for stress-related disorders.
  • Ketamine reverses reward-seeking and coping deficits, while paroxetine alleviates both repetitive/avoidance behaviors and reward-seeking/coping impairments.
  • The study validates CRS as a model with face, construct, and predictive validity for translational neuroscience, offering standardized protocols for chronic stress research.