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Phase separation of an FtAT-hook transcription factor regulates seed development under heat stress in Tartary buckwheat - PubMed

7 hours ago
  • #Phase separation
  • #Tartary buckwheat
  • #Heat stress
  • Heat stress threatens crop production and food security, necessitating the identification of thermotolerance genes.
  • A Tartary buckwheat FtAT-hook transcription factor undergoes phase separation, negatively regulating seed development under heat stress.
  • FtAT-hook increases the expression of sugar transporter gene FtERDL6 and flavonoid synthesis gene FtFLS.
  • FtASR (abscisic acid stress-ripening protein) interacts with FtAT-hook, influencing its phase separation under heat stress.
  • Kaempferitrin, a downstream product of FtFLS, suppresses FtAT-hook phase separation via negative feedback.
  • Exogenous kaempferitrin enhances FtASR-FtAT-hook interaction, improving seed size under heat stress.
  • Findings suggest flavonoid compounds can modulate protein phase separation, offering strategies to enhance crop resilience.