Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis - PubMed
12 hours ago
- #Osteoarthritis therapy
- #Energy metabolism
- #Artificial mitochondria
- Artificial mitochondria (CAMs) were developed to restore cellular energy metabolism homeostasis in osteoarthritis (OA).
- CAMs consist of crosslinked phosphocreatine monomers (MPCr) and perfluorooctyl acrylate, designed for targeted delivery and resistance to biofluid interference.
- CAMs enter degenerated chondrocytes and meniscus fibrochondrocytes via clathrin-mediated endocytosis, escape lysosomal degradation, and scavenge reactive oxygen species (ROS).
- CAMs restore ATP production, upregulate chondrogenic markers (COL2A1, ACAN, SOX9), and suppress inflammatory pathways (MMP3, IL6).
- In a murine OA model, CAM injections reduced synovial inflammation, preserved cartilage glycosaminoglycan content, and restored gait function.
- Histological and radiographic assessments confirmed CAMs mitigated joint space narrowing and cartilage erosion.
- CAMs offer a mitochondria-agnostic platform for treating degenerative diseases by rectifying cellular energy imbalance.