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Aspartate availability drives differential engagement of the malate-aspartate shuttle - PubMed

3 hours ago
  • #mitochondrial metabolism
  • #aspartate
  • #malate-aspartate shuttle
  • The malate-aspartate shuttle transfers reducing equivalents from the cytosol to mitochondria.
  • Aspartate availability determines the engagement of the malate-aspartate shuttle in cells.
  • Increasing aspartate availability enhances the use of the shuttle and mitochondrial metabolism of glucose-derived pyruvate.
  • During differentiation, elevated flux through the shuttle enables fueling of mitochondrial networks from glucose-derived carbon.
  • Engineering aspartate demand can reverse metabolic signatures of differentiated cells.
  • Cell-state-specific aspartate demand influences shuttle use and mitochondrial substrate preferences during differentiation.