Ablation of PKCα Phosphorylation by CRISPR-Cas9 Base Editing Rescues Heart Failure - PubMed
3 days ago
- #Heart Failure
- #PKCα Phosphorylation
- #CRISPR-Cas9
- PKCα phosphorylation at T497 plays a key role in heart failure pathogenesis.
- CRISPR-Cas9 base editing was used to create a phospho-resistant PKCα (T497A) mutation in mice.
- Mice with the T497A mutation showed protection against heart failure-related cardiac dysfunction and remodeling.
- Adeno-associated virus 9 delivered base editing to introduce T497A in wild-type mice, showing similar cardioprotective effects.
- Human induced pluripotent stem cell-derived cardiomyocytes with PRKCAT497A were protected from AngII-induced impairments.
- Editing PRKCAT497A via adenine base editing represents a potential therapeutic strategy for heart failure.