Gui Shen Wan ameliorates PCOS-like cellular phenotypes by suppressing TNF-α-mediated inflammation and restoring the PI3K/Akt signaling pathway - PubMed
5 hours ago
- #PCOS
- #Inflammation
- #Traditional Chinese Medicine
- Gui Shen Wan (GSW) ameliorates PCOS-like cellular phenotypes by targeting TNF-α-mediated inflammation and restoring PI3K/Akt signaling.
- Network pharmacology and in vitro studies using KGN cells under metabolic stress were employed to investigate GSW's mechanisms.
- GSW-medicated serum improved cell viability, reduced apoptosis, and decreased inflammatory cytokines (TNF-α, IL-6).
- GSW enhanced PI3K and Akt phosphorylation, with TNF-α rescue experiments confirming its mechanistic role.
- Key constituents (embelin and nobiletin) replicated GSW's anti-inflammatory and signaling effects, showing enhanced combined activity.
- The study suggests GSW's potential in mitigating PCOS-related granulosa cell dysfunction via TNF-α suppression and PI3K/Akt pathway restoration.