Dual-layer N-glycoproteomics enhances resolution of serum glycosylation signatures in type 2 diabetes mellitus complicated with coronary heart disease - PubMed
3 hours ago
- #Coronary heart disease
- #Type 2 diabetes
- #N-glycoproteomics
- Development of an integrated dual-layer N-glycoproteomic strategy combining high-coverage site-specific N-glycosylation profiling with intact N-glycopeptide analysis.
- Application to serum samples from healthy controls, T2DM, CHD, and T2DM&CHD patients, identifying 497 N-glycosites from 781 site-specific N-glycopeptides and 133 N-glycosites from 341 intact N-glycopeptides.
- Revealed extensive glycoproteome remodeling in T2DM&CHD, characterized by increased microheterogeneity due to altered sialylation and fucosylation.
- Site-specific changes in apolipoprotein B (N185, N1523, N2239, N3411) showed stage-dependent patterns linked to lipid dysregulation and vascular injury.
- Functional enrichment suggests T2DM amplifies inflammation-related glycosylation disturbances predisposing to cardiovascular complications.
- Correlation with glycosyltransferase expression highlights regulatory relevance of glycan biosynthesis.
- Provides a mechanistic resource for biomarker discovery in metabolic-cardiovascular comorbidity.