Efficient multiplex non-viral engineering and expansion of polyclonal γδ CAR-T cells for immunotherapy - PubMed
3 hours ago
- #immunotherapy
- #gamma delta T cells
- #CAR-T cells
- Gamma delta (γδ) T cells recognize non-peptide, non-MHC-associated antigens on transformed and infected cells.
- γδ T cells are being developed as an off-the-shelf cellular immunotherapeutic due to their unique properties and inability to cause GvHD.
- Challenges in γδ T cell research include low abundance, heterogeneity, limited expansion methods, and under-developed genetic modification techniques.
- A feeder-free, scalable method for ex vivo manufacture of polyclonal, non-virally modified CAR-γδ T cells is presented.
- Engineered CAR-γδ T cells show strong functionality in vitro and in vivo.
- In vivo pharmacokinetic profiling reveals subset-specific responses to IL-15 cytokine armoring and multiplex base editing.
- The study provides a platform for genetic modification of polyclonal CAR-γδ T cells and explores their therapeutic efficacy.