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Time-resolved functional genomics using deep learning reveals global hierarchical control of autophagy - PubMed

4 hours ago
  • #deep learning
  • #functional genomics
  • #autophagy
  • Study combines time-resolved high-content imaging, deep learning, and latent feature analysis to map the genetic network controlling autophagy activation and inactivation during nitrogen changes.
  • Dataset AutoDRY categorizes 5,919 mutants based on nutrient response kinetics and their roles in autophagosome formation and clearance.
  • Integration with functional and genetic network data reveals hierarchical and multilayered control of autophagy, uncovering new regulatory pathways.
  • Identifies the retrograde pathway as a key time-varying modulator that regulates core autophagy genes and plays a central role in autophagy inactivation.
  • Provides a systems-level resource for future autophagy research.