Defining the reference proteomes for small extracellular vesicles and non-vesicular components - PubMed

6 days ago

Extracellular vesicles (EVs) are important for intercellular communication and as biomarkers.
Molecular characterization of EVs is challenging due to heterogeneity and co-isolated non-vesicular components.
Protein correlation profiling was used to analyze over 9,000 proteins in human cancer cell lines and biofluids.
Small EVs (sEVs) primarily incorporate plasma membrane proteins through selective cargo-loading mechanisms.
Cytosolic proteins in sEV preparations are externally associated, likely from cellular debris and aggregates.
The study provides a reference resource for understanding sEV biogenesis and cargo selection.

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