Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD - PubMed
a day ago
- #single-cell analysis
- #liver disease
- #MASLD
- Single-cell analysis identified novel intermediate cell populations in severe MASLD.
- Study used Sc-RNAseq and spatial proteomics to analyze cellular heterogeneity in MASLD progression.
- Identified 32 cell clusters, including hepatocytes, stellate cells, endothelial cells, and immune cells.
- Hepatocyte clusters showed changes correlating with disease severity and periportal localization in fibrosis.
- Advanced disease featured endothelial cells with high ECM molecule expression and M1-polarized immune cells.
- Five hybrid cell populations (e.g., HSCs/ENDOs, HEPs/KCs) were confirmed in both mice and human MASLD patients.
- Findings highlight cellular evolution and novel intermediate populations in advanced MASLD.