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Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature - PubMed

3 hours ago
  • #microfluidics
  • #cancer-diagnosis
  • #extracellular-vesicles
  • Small extracellular vesicles (sEVs) provide a non-invasive method for early-stage multi-cancer detection.
  • A 4-protein sEV biomarker panel (thrombospondin-1, nidogen-1, pentraxin-3, versican) was identified and validated across 22 cancer cell lines and 764 plasma/serum samples with high accuracy (AUC: 0.91-1.00).
  • A multiplex sEV device combining nanoshearing microfluidics and SERS was developed for clinical application, enabling simultaneous detection of the 4-protein panel.
  • The device successfully differentiated benign lung changes from early-stage lung cancer in a prospective cohort of 68 patients.
  • The study highlights the scalability, simplicity, and cost-effectiveness of the multiplex microfluidic device for large-scale cancer screening.