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Deciphering the Role of Cancer Stem Cells in Breast Cancer Brain Colonization Using a Novel Patient-Derived Model - PubMed

7 hours ago
  • #Brain Metastasis
  • #Cancer Stem Cells
  • #Breast Cancer
  • A novel patient-derived model isolates cancer stem-like cells (CSCs) from human breast cancer brain metastases (BCBM) and characterizes their stem-like properties.
  • These CSCs demonstrate the ability to generate brain and bone metastases in xenograft models, recapitulating patient-specific dissemination patterns.
  • A metastasis-initiating cell (MIC) subpopulation within CSCs is identified, enriched in stemness and adhesion pathways, and shows enhanced brain-specific transcriptomic reprogramming.
  • MICs enable vascular co-option, resist stromal stress, and interact with brain-resident cells to promote survival, highlighting their key role in brain colonization.
  • High-throughput drug screening reveals broad therapeutic resistance in the CSC compartment, emphasizing the need for targeted strategies against CSCs and MICs.