Distinct spatial patterning and transcriptomic landscapes of human neural organoids by localized delivery of morphogens - PubMed
4 hours ago
- #spatial patterning
- #neural organoids
- #morphogen gradient
- Human neural organoids were studied for spatial patterning and transcriptomic landscapes using localized morphogen delivery.
- A Matrigel-free passive diffusion-based morphogen gradient generator (PdMG) was developed to create stable gradients in neural organoids.
- Different morphogen gradients (Sonic hedgehog/WNT inhibitor, WNT, retinoic acid) induced dorsal-ventral and rostral-caudal patterning in forebrain, midbrain, and hindbrain-like organoids.
- Spatial transcriptomics showed robust regionalization in early-stage organoids and active neurogenesis and GABAergic interneuron migration in late-stage organoids.
- The study provides a framework for modeling spatial-temporal morphogen dynamics in neural organoids.