A Bioluminescence Reporter Mouse Strain for In Vivo Imaging of IFNγ Cell Localization and Function - PubMed
8 hours ago
- #IFNγ
- #CRISPR/Cas9
- #Bioluminescence Imaging
- Development of a C57BL/6J-Ifng-2A-luciferase knock-in mouse strain using CRISPR/Cas9 for real-time bioluminescence imaging (BLI) of IFNγ-expressing cells.
- The model enables direct detection of Poly(I:C)-induced transient IFNγ and enhanced intratumoral IFNγ signals during anti-PD-1/CTLA-4 therapy.
- Dynamically tracks IFNγ expression in imiquimod-induced psoriasis, providing insights into IFNγ-mediated immune regulation in inflammation and cancer.
- Offers potential for identifying IFNγ-related therapeutic targets and predicting immunotherapy responses.