CRISPR gene editing of AtRING1 unravels a critical role of RAWUL domain in PRC1 repression of transcription - PubMed
3 hours ago
- #Epigenetics
- #Plant Development
- #CRISPR
- CRISPR gene editing reveals the critical role of the RAWUL domain in AtRING1 for PRC1-mediated transcriptional repression.
- AtRING1 is a key component of PRC1 in Arabidopsis, featuring an N-terminal RING-finger domain and a C-terminal RAWUL domain.
- The study generated AtRING1 mutants, including an N-terminal stop mutant (atring1ko) and C-terminal deletion mutants (atring1ΔC-terminal), to investigate domain functions.
- The atring1ko mutant showed severe developmental defects, indicating AtRING1's essential role in cell differentiation.
- C-terminal deletion mutants exhibited milder defects, suggesting the RING domain alone retains partial function, while the RAWUL domain fine-tunes PRC1 activity.
- Molecular analyses indicate that AtRING1/PRC1-mediated H2A monoubiquitination (H2Aub1) often precedes PRC2-mediated H3K27me3 deposition at target loci.
- The RAWUL domain is crucial for efficient H2Aub1 enrichment and influences H3K27me3 deposition in a locus-specific manner.
- Findings provide new insights into PRC1's E3 ligase activity and its role in facilitating PRC2 activity in epigenetic gene silencing.