Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cells - PubMed
3 hours ago
- #genome profiling
- #mammalian cells
- #DNA replication
- TrAEL-seq is a method for profiling DNA replication genome-wide in unsynchronized cells without drugs or nucleotide analogues.
- An updated TrAEL-seq protocol improves sample quality and allows multiplexing of up to six samples, enhancing throughput.
- The method provides high-resolution replication profiles comparable to OK-seq in mammalian cell lines.
- TrAEL-seq is effective in dynamic systems, such as during oncogene-induced senescence.
- It maps replication initiation and termination zones and is sensitive to replication fork speed.
- Replication forks slow down in transcribed regions but this does not broadly affect replication dynamics.
- Forks accelerate over the first ~1 Mb of travel, regardless of local transcription.
- Fewer active forks later in S-phase and less competition for replication factors may explain this acceleration.