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RAD51C-XRCC3 complex regulates FANCM-mediated R-loop resolution to safeguard genome integrity - PubMed

9 hours ago
  • #RAD51C-XRCC3 complex
  • #FANCM
  • #R-loop resolution
  • The RAD51C-XRCC3 (CX3) complex plays a role in the Fanconi anemia (FA) pathway by regulating R-loop tolerance mechanisms.
  • CX3 complex suppresses R-loops, transcription-replication collisions (TRCs), and associated genome instability under physiological and replication stress conditions.
  • The CX3 complex physically interacts with FANCM, facilitating its recruitment to R-loop sites to promote resolution.
  • Cells with the RAD51C R258H pathological mutant show defective interaction with FANCM and inefficient R-loop processing.
  • The CX3 complex-mediated R-loop resolution is independent of its fork maintenance function.
  • This study reveals a new role for the CX3 complex in preventing R-loop-induced genome instability by regulating FANCM activity.