Pathogenicity and vaccine protection of circulating pertactin- and filamentous hemagglutinin-deficient Bordetella pertussis strains - PubMed
12 hours ago
- #pathogenicity
- #vaccine protection
- #Bordetella pertussis
- Study focuses on pathogenicity and vaccine protection of Bordetella pertussis (Bp) strains deficient in pertactin (PRN-) and filamentous hemagglutinin (FHA-).
- PRN- isolates showed similar proliferation, adhesion, and invasion to wild-type (WT) but induced stronger pro-inflammatory responses and increased cell death.
- FHA- and FHA-PRN- isolates grew faster in vitro but had reduced adhesion, invasion, and THP-1 cell death, with similar lung colonization to WT.
- PRN- induced a WT-like lung immune profile, while FHA- and FHA-PRN- led to higher neutrophil proportions and reduced alveolar macrophages/dendritic cells.
- DTcP and DTaP vaccines provided varying protection levels, with breakthrough infections in 69.57% of mice challenged with FHA-PRN- strains.
- Findings suggest PRN and FHA deficiencies alter virulence phenotypes and immune responses, impacting vaccine efficacy.