Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine - PubMed
3 hours ago
- #biomolecular condensates
- #extracellular granules
- #mast cells
- Mast cell extracellular granules (MCEGs) are bioactive condensates formed by electrostatic interactions between glycosaminoglycans (heparin) and polyamines.
- Disrupting polyamine synthesis or trafficking inhibits MCEG formation and affects protease and cytokine storage.
- Reconstituted granules with heparin and spermine enrich mediators like carboxypeptidase A3 (CPA3) and tumor necrosis factor (TNF), maintaining unique biochemical conditions (elevated pH, higher calcium/zinc).
- MCEGs enhance CPA3 enzymatic activity and TNF bioactivity toward endothelial cells.
- MCEGs represent a novel type of biomolecular condensate with distinct biochemical and immunological properties, expanding condensate assembly mechanisms beyond protein-protein/RNA interactions.