Single-cell spatial map of cis-regulatory elements for disease-related genes in the macaque cortex - PubMed
4 hours ago
- #cis-regulatory elements
- #single-cell
- #primate cortex
- Single-cell spatial transcriptomes reveal molecular and cellular diversity in the brain.
- Single-nucleus ATAC-seq performed on ~1.6 million cells from 142 cortical regions of two male cynomolgus monkeys.
- Identified distinct chromatin accessibility profiles of cis-regulatory elements (CREs) for various cell types.
- CREs showed laminar and regional preferences, with regional accessibility dependent on the region's hierarchical level.
- Cross-species comparison revealed human/macaque-enriched neurons and biased CREs for neurodevelopment and psychiatric disease genes.
- Risk SNPs for brain disorders associated with human/macaque-biased CREs in glutamatergic neurons and microglia.
- Provides basis for understanding gene regulatory mechanisms in primate cortex cellular diversity and disease pathogenesis.