Potential effects of endogenous RNA/DNA hybrids on CRISPR-Cas9-mediated homology-directed repair - PubMed
7 hours ago
- #Homology-directed repair
- #CRISPR-Cas9
- #R-loops
- R-loops, endogenous RNA/DNA hybrids, may influence CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency.
- In proliferating hepatocyte-derived cells, HDR efficiency is reduced at R-loop-enriched sites, but not in quiescent hepatocytes in vivo.
- Reducing R-loop levels via RNaseH1 overexpression or G1 arrest induction increases HDR efficiency at these sites.
- T cell activation correlates with elevated R-loop accumulation, suggesting potential impacts on ex vivo genome editing.
- Indel frequencies remain comparable across sites with different R-loop abundance, indicating specificity to HDR effects.