A correlative workflow for synaptic imaging by cryo-electron tomography - PubMed
4 hours ago
- #correlative microscopy
- #synaptic imaging
- #cryo-electron tomography
- A correlative workflow combining cryo-light and electron microscopy targets synapses for cryo-electron tomography imaging.
- Synapses are fluorescently labeled using a synthetic binder for postsynaptic PSD-95 and antibodies for presynaptic synaptotagmin-1.
- Cryo-focused ion beam milling thins cellular samples, guided by fluorescence to enable high-quality cryo-ET imaging at synaptic sites.
- Cryo-ET at colocalization sites primarily identifies excitatory synapses, facilitating in situ structural studies of synapse-resident protein complexes.