Porcine epidemic diarrhea virus infection activates SREBP2 and induces RORγ expression to enhance cholesterol biosynthesis and virus replication - PubMed
2 days ago
- #Viral replication
- #PEDV
- #Cholesterol biosynthesis
- PEDV infection activates SREBP2 and induces RORγ expression to enhance cholesterol biosynthesis.
- Cholesterol is essential for PEDV replication, and its biosynthesis is upregulated by PEDV via SREBP2 and RORγ.
- Silencing RORγ or using inhibitors (XY018, GSK805) reduces cholesterol synthesis and impedes PEDV replication.
- RORγ overexpression enhances cholesterol biosynthesis-related gene expression and viral replication.
- Co-inhibition of SREBP2 and RORγ synergistically suppresses PEDV replication.
- Exogenous cholesterol supplementation counteracts the inhibitory effects of RORγ inhibitors on virus replication.
- PEDV-induced RORγ expression depends on TAK1 and its downstream kinases JNK and IKK, activating AP-1 and NF-κB signaling.
- The study identifies the TAK1-JNK/IKK-RORγ axis as critical for cholesterol biosynthesis and PEDV replication.