Immune evasive DNA donors and recombinases license kilobase-scale writing - PubMed
12 hours ago
- #genome-editing
- #recombinases
- #immune-evasion
- Immune evasive DNA donors and recombinases enable kilobase-scale genome writing.
- Traditional methods use large double-stranded DNA (dsDNA) donors, which trigger toxic immune responses.
- INSTALL (Integration through Nucleus-Synthesized Template Addition of Large Lengths) uses circular single-stranded DNA (cssDNA) to evade immune detection.
- INSTALL improves integration efficiency and tolerability in primary human cells and mice.
- The method supports systemic in vivo non-viral DNA delivery with broader dosing ranges.
- INSTALL synergizes immune-stealth nucleic acids with recombinases, eliminating the need for viral vectors.
- The technology is compatible with diverse protein and RNA-guided recombinases.
- Several authors have filed patents related to INSTALL and genome engineering technologies.
- Some authors have financial interests or consulting roles in biotech companies developing gene-editing therapies.