Conformational biosensors delineate endosomal G protein regulation by GPCRs - PubMed
4 days ago
- #endosomal signaling
- #G protein regulation
- #GPCR
- Conformational biosensors reveal how GPCRs regulate G proteins in endosomes.
- Three Gs-coupled GPCRs (β2-adrenergic receptor, VIP-1 receptor, adenosine 2B receptor) redistribute Gαs from plasma membrane to endosomes without receptor internalization.
- Active-state Gαs production on endosomes depends on GPCR internalization.
- GPCRs show location bias in coupling selectivity between Gs and Gq on endosomes vs. plasma membrane.
- Endosomal Gs regulation involves two discrete coupling reactions: one at the plasma membrane controlling Gs concentration, another at endosomes controlling Gs activity.
- GPCR endocytosis can switch signaling selectivity between G protein classes.