Targeted intracellular oral RNA delivery through tea polyphenol nanovesicle to outer membrane vesicle transfer for colitis treatment - PubMed
7 hours ago
- #nanovesicle technology
- #colitis treatment
- #oral RNA delivery
- The study developed a vesicle-to-vesicle transfer strategy for efficient oral RNA delivery using tea polyphenol nanovesicles and outer membrane vesicles (OMVs) from E. coli Nissle 1917.
- RNA was encapsulated in nanovesicles formed by acylated epigallocatechin, then transferred to OMVs via membrane fusion to create hybrid vesicles (HVs) for targeted delivery.
- HVs were further functionalized with bilirubin and a HA-BR-KDEL ligand to sequentially target inflammatory cells and the endoplasmic reticulum in the inflamed intestine.
- Oral administration of the engineered HVs significantly alleviated colitis symptoms in mice and promoted restoration of intestinal homeostasis.